I think you can make it out only if you play it at home with no other extraneous noise / music and only if you listen closely. Once you're a part of a mix - too much going on to latch onto such details.
Years back, when I played bass on a lounge gig, I played the house Gibson bass which had a sliding pickup. The difference was noticeable when you moved from the neck to the bridge but then again, smaller shifts didn't really have that much incremental difference.
A lot seem to harp on the harmonic mode at the 24th fret location, where a pickup would be placed on a 21/22 fret guitar, but that logic is flawed - that node changes the minute one frets a string.