I A L W A Y S use a tuner. As should everyone at the gig, session, etc.
I'm a tuning fanatic, I wouldn't give you 2 cents for a bass that won't stay in tune. I always looked at it from the standpoint that since technique and tone were constantly moving targets, I certainly had no patience left to chase tuning during a gig.
I only chime harmonics, tuning-wise, for a quick check between tunes. On my five-string, I fell into this quick, across the neck routine:
Chime the G and D string at the 7th fret, then play the note at the fifth fret on the A string: This will form a basic D chord (d-a-d).
Repeat this shape one string over, you get the idea. I spell out these three chords on my five (or 2 on a 4 string, 4 on a 6, etc.) just to check.
I'm sure there are those here who know LOTS more harmonic chords than I do that would serve as well.
Typically, I aim to have basses that I can actually take back to the gig tomorrow night that are still in tune from today, and usually I'm pretty close. I ONLY synchronize my harmonics when the new strings are installed. I NEVER leave the bass in the car to get too hot or too cold. I am really picky about winding them on the posts properly. I make sure the nut and bridge slots are free. I keep the tension adjusters in the keys firm, but not over tight.
And fortunately, neck thrus eliminate the bolt joint, thereby eliminating one big out of tune potential.
I'm past my bass-banging days, but I don't baby them. And they stay in tune.
NEVER let you guitar player tune it !
J o e y