I started on a lined fretless neck, and that helped me gain confidence early on. I now play 34 and 30.75 electrics, both of which aren't lined. Each bass uses dots to indicate something different: on fret/center of fret. Furthermore, the 3,5,7,9,12,etc,etc dots on my Alembic are LEDs with no silver barrel to outline them~ so sometimes when it's not plugged in, I'll look down and aim for a dot, and be a half step off!
I never bothered taking a tuner and pencil to my upright and hashing out spots, and to this day I always flinch when going to the 3rd position, so even though I try not to rely on side dots/lines, subconsciously I probably do.